Poster C39, Sunday, March 25, 1:00-3:00 pm, Exhibit Hall C
The circuit analyses of Anesthesia-resistant memory in Drosophila.
Emmanuel Antwi-Adjei1, Diana Hilpert2, Martin Schwärzel1; 1Free University Berlin
The memory consolidation theory classifies memory in accordance to whether it resists an amnestic treatment, or not. The Drosophila aversive odour-learning is a Pavlovian training where flies are trained to avoid an olfactory cue- the conditioned Stimulus- after it has been presented in combination with an electric shock. However, the Drosophila aversive learning are composed of two different types of consolidated memories, i.e., Long-term memory and anesthesia-resistant memory. Also, in contrast to LTM, anesthesia-resistant memory is induced by single cycle and it forms an integral part of mid-term memory . Furthermore, presynaptic proteins have been shown to be pivotal for distinct memory phases. Bruchpilot, a presynaptic protein was revealed to be essential for olfactory memory, and specifically affected Anesthesia-resistant memory at the level of mushroom body (Knapek et al., 2011). In addition to this, Bruchpilot is homologous to the mammalian ELKS/CAST family of active zone proteins, which is specifically localized to the presynaptic dense bodies (Wagh et al., 2006). Henceforth, the focus of this research project was to investigate the role of Bruchpilot on the olfactory pathways in the Drosophila. This was done by performing a transgenic knockdown of this presynaptic protein at the level of olfactory receptor neurons , antennal lobe, mushroom body, dopaminergic neurons and extrinsic neurons. The outcome of this research project revealed that the antennal lobe local interneurons 2 was the main target for the encoding of the anesthesia resistant memory and was phase locked with the mushroom body neurons and extrinsic neurons.
Topic Area: EXECUTIVE PROCESSES: Working memory