Poster C114, Sunday, March 25, 1:00-3:00 pm, Exhibit Hall C
Suppressing lateral orbitofronto-striatal pathway improved the learning of delayed paired associative learning task in mice
Cheng Qi1,2, Hou Rui Qing1, Chen Zhao Qin1, Fan Hong Mei1, T. Li Chengyu1; 1Institute of Neuroscience and Key Laboratory of Primate Neurobiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China, 2University of Chinese Academy of Sciences, Beijing 100049, China.
Working memory(WM) was an important cognitive function for our daily life. However, little was known about how to improve it when we were engaged in this cognitive process. To study this question, Mice were trained to learn an olfactory delayed paired associative learning task(DPAL). During the delay period mice must actively keep in mind the odor identity to successfully perform the task for water reward. By suppressing the lateral orbitofrontal(LO) cortex during delay with optogenetics, mice could learn the DPAL task better with blind designs. With extracellular recordings, we found that silencing LO could not improve the population coding ability of LO neurons for odor identity, which proposed the question about the mechanisms of the improved performance observed by suppressing LO. Based on the hypothesis of the distributed nature of working memory, we postulated that suppressing LO could works through its downstream pathways. We found that LO-striatum terminal inhibition with optogenetics mimicked the effects of the suppression of LO per se, while not the LO-vertral tegmental area, LO-perirhinal cortex and LO-secondary motor cortex axonal terminal inhibition. Our study gave some hints of the understanding of how to improve working memory, and the possible neural networks involved in this process.
Topic Area: EXECUTIVE PROCESSES: Working memory