Poster D15, Monday, March 27, 8:00 – 10:00 am, Pacific Concourse
Impact of acute lung injury on cognitive function in experimental mice
Amarjit Naura1, Bijayani Sahu1, Rajat Sandhir1; 1Department of Biochemistry, Panjab University, Chandigarh
Acute lung injury (ALI) is a life threatening disorder characterized by accumulation of large numbers of neutrophils in the lungs. Interestingly, ALI survivors frequently present some cognitive deterioration at discharge; such as impaired memory, attention, concentration and/or mental processing speed. However, the molecular mechanism behind ALI mediated cognitive function is not known. Accordingly the present work was designed to investigate potential impact of ALI on cognitive impairment in mice for its potential application as a model system to study cross talk between lung and brain at molecular level. ALI in male Balb/c mice was induced by intra-tracheal administration of either HCl or LPS as single hit or both agents were administered to mimic ‘two hit’ model of ALI. Administration of either LPS or HCl alone increased the neutrophils in lungs significantly but did not impair memory as assessed by Morris water maze test. Interestingly ‘two hit’ mediated injury resulted in more pronounced increase in neutrophils and pro-inflammatory factors in lungs which was found to be associated with persistent decline in memory. It appears that exaggerated lung injury through two hits disrupt the threshold barrier to cause cognitive dysfunction. Indeed further analysis revealed that two hit mediated injury lead to disruption of blood brain barrier potentially through elevated systemic inflammatory response. Overall, our findings show that two hit mediated ALI leads to cognitive impairment in mice and hence the model can be used to examine lung-brain cross talk at molecular level.
Topic Area: ATTENTION: Spatial